Roma finished above Chelsea to avoid Barcelona, instead landing a tie against the Ukrainians.
Meanwhile, Shakhtar edged out Napoli to finish behind Manchester City in Group F.
Both teams will be confident of progressing into the quarters.
Cengiz Ünder is surely one of the first names on the Roma teamsheet on current form and should make his Champions League bow here.
Diego Perotti and Stephan El Shaarawy are in competition for a starting berth on the left flank for Roma.
Daniele De Rossi and Lorenzo Pellegrini compete for one starting spot in central midfield alongside Kevin Strootman.
Bruno Peres served a one-match ban in Roma's final group game but is free to play here.
The away side will be without Rick Karsdorp and Maxime Gonalons on Wednesday.
Maksim Malyshev is the only injury concern for Shakhtar Donetsk at the moment. Expect few changes from the side that qualified from the same group as Manchester City.
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